We evaluated several approaches for accelerating inbreeding, including the. A recombinant inbred strain or recombinant inbred line is an organism with chromosomes that incorporate an essentially permanent set of recombination events between chromosomes inherited from two or more inbred strains. Vitamin e ve is an important nutritional trait in rice grains. Predicting the properties of recombinant inbred lines.
In this paper we present a novel approach to quantifying genetic architecture that combines recombinant inbred lines ril with line cross analysis lca. In all, 37 qtl were detected in the two populations. As each ril is an inbred strain, and so can be propagated eternally, a panel of rils has a number of advantages for genetic. A total of 99 recombinant inbred lines rils generated from a cross between oriental azumamugi and occidental type kanto nakate gold of barley hordeum vulgare l. After 20 generations of inbreeding, the new strains can be registered, and. We consider recombinant inbred lines obtained by crossing two given homozygous parents and then applying multiple generations of selfcrossings or fullsib. The genomes of recombinant inbred lines 2 inbred mice. Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean author. Genomic regions governing the biosynthesis of unsaturated. Recombinant inbred ri lines are particularly useful in genetic mapping studies of quantitative traits. We use a rapid cycling strategy to attempt to advance 1. Reinventing potato as a diploid inbred linebased crop.
Quantitative trait loci associated with micronutrient. Each disease was scored in multiple separate trials. Resistance to beet armyworm in a chickpea recombinant. Generating inbred lines via sibling matings, however, is a costly undertaking that requires many successive generations of breeding, during which time many lines fail. Development and screening of cowpea recombinant inbred. Accelerating the inbreeding of multiparental recombinant. Agronomic and molecular evaluation of recombinant inbred. Although a handful of isolated recombinant inbred ri mouse strains were developed during the 1940s and 1950s, the importance of these genetic constructs was not appreciated by any scientist prior to dr.
Totally, 29 qtls for six ve traits were identified in 2 consecutive years. A recombinant inbred population was derived from the glycine max l. Recombinant inbred strains, such as the bxd strain set, are significantly advantageous for the complex trait. Seventyone ri iines of the f 6 and f 7 generations were used for rflp mapping. Realizing the full potential of these crosses requires computational tools capable of handling the increased statistical complexity of the analyses. Mccouch, s r and doerge, r w 1995 qtl mapping is rice trends genet 11,482487. Broman1 department of biostatistics, johns hopkins university, baltimore, maryland 21205 manuscript received august 20, 2004 accepted for publication november 5, 2004 abstract recombinant inbred lines rils can serve as powerful tools for genetic mapping. Individual lines in two of seven populations evaluated by dropmycelium method were significantly different p recombinant inbred lines rils from as many genetically divergent germplasm sources as practical to examine trait genetics. Ostrinia nubilalis ecb and sesamia nonagrioides mcb are two maize stem borers which cause important losses in temperate maize production, but qtl analyses for corn borer resistance were mostly restricted to ecb resistance and maize materials genetically related mapping populations derived from b73.
Download fulltext pdf the use of recombinant inbred lines rils for genetic mapping article pdf available in methods in molecular biology 82. Quantitative trait loci qtls and epistasis for arabidopsis thaliana aluminum al tolerance were analyzed using a recombinant inbred ri population of 100 lines derived from a cross between landsberg erecta and columbia col. Design and construction of recombinant inbred lines. Design and construction of recombinant inbred lines article pdf available in methods in molecular biology clifton, n. Pdf design and construction of recombinant inbred lines. Genetic linkage map of a recombinant inbred lines in tomato.
The genomes of recombinant inbred lines complex trait. Inbred model organisms are powerful tools for genetic studies because they provide reproducible genomes for use in mapping and genetic manipulation. Pdf the use of recombinant inbred lines rils for genetic. In this study, the resistance locus rpshn in the soybean line meng8206 was mapped using two mapping populations. The mouse collaborative cross cc is a panel of eightway recombinant inbred lines. We describe a recombinant inbred line ril population of 161 f5 genotypes for the widest euploid cross that can be made to cultivated sorghum sorghum bicolor using conventional techniques, s. Wed like to understand how you use our websites in order to improve them.
Multiparent crosses of recombinant inbred lines provide opportunity to map markers and quantitative trait loci qtl with much greater resolution than is possible in biparental crosses. Distribution of parental genome blocks in recombinant inbred lines. Recently, members of the complex trait consortium proposed the development of a large panel of eightway rils in the mouse, derived from eight genetically diverse parental strains. The afrc funded the commercial bulking of these lines. Recombinant inbred lines how is recombinant inbred lines. The bulked f8 seeds we are distributing from the first 100 lines were supplied to us by the nottingham arabidopsis stock centre.
Crop science abstract genetic mapping of agronomic. The use of such eightway rils will require a detailed understanding of the. Recombinant inbred ri strains are formed by crossing two inbred strains and obtaining a genetically segregating population, perhaps an f 2 or more advanced hybrid cross, then establishing new inbred lines, each from a single breeding pair. An inbred line consists of individuals with the same genotype. Main plots included two irrigation treatments of 70 and 140 mm evaporation. Mapping resistance quantitative trait loci for three. Predicting the properties of recombinant inbred lines derived by single seed descent. Data on morphological traits were collected and analyzed. Wedding recombinant inbred lines with traditional line. Recombinant inbred lines for genetic mapping in tomato springerlink. Therefore, the objective of this work was to identify and characterize qtls for mcb. Phenotypic data are cumulative for each inbred line and. Genetic variation for agronomic characters and drought. These ril were genotyped using 105 polymorphic ssr markers across soybean genome and analyzed for fatty acid composition.
For mouse, the lxs panel of recombinant inbred strains williams et al. Larvae feed on the vegetative and reproductive stages of chickpea and the development of plant resistance is a priority in the management of this pest. Frontiers fine mapping of a resistance gene rpshn that. Parent strains are crossed to create recombinants that are then inbred to isogenicity, resulting in a permanent resource for trait mapping and analysis. However, there are several advantages to ultimately generating inbred lines maintained as tps rather than tubers. Pdf quantitative trait loci controlling agronomic traits. F1 and f2 generations are produced by intercrossing the inbred strains. Nearly 800 quantitative trait loci qtls with major and 33 minor effects were identified as contributing to the phenotypic variation of plant 34 architecturerelated traits.
Recombinant inbred ri strains should be designated by upper case one or twoletter. Phytophthora root rot prr, caused by phytophthora sojae, has negative effects on soybean yield in china and can be controlled by identifying germplasm resources with resistance genes. Signatures of dobzhanskymuller incompatibilities in the genomes. Directional genetic effects are thought to be critical components of genetic architecture for the long term. The use of such eightway rils will require a detailed understanding of. Recombinant inbred ri iines of rice oryza sativa l. Such a panel would be a valuable community resource. True potato seed can be easily shared among research programs, each of which is likely to collect phenotypic data on a different set of traits. Qtl analysis of seed dormancy in arabidopsis using recombinant inbred lines. The use of recombinant inbred lines rils for genetic.
Reduce individual variation by phenotyping multiple individuals from each strain. Initial mapping was realized using two recombinant inbred line ril populations and included 103. Recombinant lines obtained from an interspecific cross between lycopersicon species selected by fruit weight and fruit shelf life. Jiang, guoliang, wang, xianzhi, green, marci, scott, roy, hyten, david, cregan, perry source. Recombinant inbred lines rils are a collection of strains that can be used to map quantitative trait loci. The objective of this study was to select drought tolerant cowpea recombinant lines developed from a drought tolerant and susceptible parent using the wooden box screening technique. Recombinant inbred strain an overview sciencedirect topics. Donald bailey who first conceptualized their potential utility for linkage analysis while. Localization of qtls for seed color using recombinant inbred lines of brassica napus in different environments.
Recombinant inbred lines by sibling mating 4 advantages of ri lines each strain is an eternal resource. Many agronomic traits of interest to plant breeders are quantitative. Recombinant inbred ri strains have been used to map a wide range of mendelian loci and quantitative traits taylor 1989. Lca is a method of quantifying directional genetic effects i. Qtl analysis of al tolerance in recombinant inbred lines. Genotype probabilities at intermediate generations in the. One of the inbred populations was developed by crossing a hybrid wheat variety with a shw line, and the other was created by crossing the hybrid variety with the progeny line of the shw line. Rflp framework map constructed using the ri lines covered a distance of 1275cm containing 375 markers. This soybean population was used to investigate the. Using crossover breakpoints in recombinant inbred lines to.
An ril is formed by crossing two inbred strains followed by repeated selfing or sibling mating to create a new inbred line whose genome is a mosaic of the parental genomes figure 1. In order to dissect the genetic basis underlying ve content, a recombinant inbred lines population derived from zhenshan 97b and nanyangzhan was used for quantitative trait locus qtl analysis. Here i describe the process of designing and constructing rils. Pdf recombinant inbred lines rils are a collection of strains that can be used to map quantitative trait loci. Qtl analysis of seed dormancy in arabidopsis using. Rflp framework map using recombinant inbred lines in rice. Many generations are required to reach inbreeding, and so a number of investigators. Recombinant inbred strains, such as the bxd strain set, are significantly advantageous for.
In the construction of recombinant inbred lines rils from two. The parents were drought tolerant line crossed with susceptible line. Qtl mapping for mediterranean corn borer resistance in. Genetic characterization of a new set of recombinant inbred lines.
A recombinant inbred line population of tomato and its genetic map 443 derived from a cross between two inbred lines via selfbreeding and singleseed descent breeding approach until homozygosity or near homozygosity is reached 11. Metabolic analyses of interspecific tomato recombinant. Genetic analysis of recombinant inbred lines for sorghum. The genetic basis of plant architecture in 10 maize. Recombinant inbred lines rils can serve as powerful tools for genetic mapping.
1310 470 761 963 1504 1105 484 1545 1602 1660 105 554 469 1059 1678 355 1500 193 212 310 449 61 78 1510 546 967 1098 504 1170 1208 605 750 234 766 240 147